Abstract
Genetic polymorphisms of μ-calpain, a calcium-dependent protease specifically expressed in muscle and related to meat tenderness, have been investigated in cattle worldwide, but not well documented in Indonesian beef cattle. This study was done to evaluate the frequency of SNP 316 in the μ-calpain gene in Aceh cattle, a local Indonesian beef cattle plays important role in the fulfillment of red meat for peoples in Aceh and its neighboring areas. For this purpose, genomic DNA was isolated from 29 sirloin meats samples collected from cull, female aceh cattle slaughtered at the Slaughter House of Banda Aceh. The cattle were purposively selected based on physical characteristics and hair colors referred to Indonesian law. For the SNP genotyping, PCR-RFLP methods were set up. The results showed all three genotypes namely CC, CG, and GG were found in Aceh cattle. The allele and genotype frequencies of SNP CAPN316 in the CAPN gene were: C – 0.14 and G – 0.86; CC – 3.4%, CG – 20.7% and GG – 75.9%. The observed and expected frequencies of CAPN SNP 316 in the cattle population examined were GG 22.0 and 21.6, CG 6.0 and 6.9, and CC 1.0 and 0.6.
Highlights
Aceh cattle is Indonesian local beef cattle originated from the crossing of Bos Indicus, Bos Javanicus and Bos Sondaicus [1]
Efforts to improve genetic quality of Aceh cattle has been done by identifying phenotypic diversity of the cattle [2], determining its genetic relationship based on displacement-loop region of microsatellite DNA [1], and by establishing Indonesian National Standard criteria for
The results showed that all three calpain genotypes namely CC, CG, and GG were found in Aceh cattle
Summary
Aceh cattle is Indonesian local beef cattle originated from the crossing of Bos Indicus, Bos Javanicus and Bos Sondaicus [1]. The role is challenged by a significant decreased in population numbers, smaller body size and declined genetic quality due to inbreeding and slaughtering of productive females to fulfill market demand [7]. Efforts to improve genetic quality of Aceh cattle has been done by identifying phenotypic diversity of the cattle [2], determining its genetic relationship based on displacement-loop region of microsatellite DNA [1], and by establishing Indonesian National Standard criteria for. Whilst results of polymerase chain reactionrestriction fragment length polymorphism (PCR-RFLP) based molecular study showed novel mutation found in myostatin gene of Aceh cattle [9], no studies were done to explore diversity in the genes related to meat quality of the cattle. Among gene where nucleotide variations might influence meat tenderness, protein content and quality is calpain [10]. This study was done to identify single nucleotide polymorphism at the position of 316 of the μcalpain gene (CAPN SNP 316) in Aceh cattle have different hair colors
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