Single Nucleotide Polymorphism rs731384 is Associated with Serum Lipid Levels and the Risk of Coronary Artery Disease in Chinese Populations

Abstract

Aims: To investigate the relationship between the miR-130a polymorphism rs731384 and coronary artery disease (CAD), and to further explore the molecular mechanism of the pathogenesis of CAD, a prospective and observational single-center study was conducted. Method: 876 subjects were recruited in this study. Four millilitres of venous blood were drawn after 12 hours of fasting to perform biochemical assays. CAD patients and controls were distinguished by coronary angiography. Rs731384 was genotyped on the Agena MassARRAY system according to manufacturer's user guide. Statictical analysis was conducted with SPSS 16.0 software. Results: The study found that the serum levels of TC (p=0.006), LDL (p=0.030), ApoA (p=0.038), ApoB (p=0.022) distribute differently in various alleles. Additionally, AA genetype of rs731384 was found to be a protective factor against CAD in recessive model (AA:AG GG, OR=0.408, 95%CI=0.171-0.973, p=0.043). Significant association was found between the gene-environment interation and CAD risk. AA genetype along with HDL level ≥1.325 mmol/L significantly decreased the CAD risk (AA:AG GG, OR=0.117, 95%CI=0.023-0.588, p=0.009). Conclusion: The mutant AA genetype of rs731384 seems to be a protective factor against CAD and rs731384 plays an important role in human metabolism of serum lipid. Funding: This study received support from the National Natural Science Foundations of China (No. 81170180, 30400173 and 30971257) and the Priority Academic Program Development of Jiangsu Higher Education Institutions. Dr. En-Zhi Jia is an Assistant Fellow at the Collaborative Innovation Center for Cardiovascular Disease Translational Medicine. Declaration of Interest: There are not any conflicts of interest existing in this manuscript. Ethical Approval: The study was approved by the ethics committee of the First Affiliated Hospital of Nanjing Medical University and the Friendship Hospital of Ili Kazakh Autonomous Prefecture in China. All subjects provided written informed consent.