Single Nucleotide Polymorphism on Exon 2 Leptin Gene of Pasundan Cattle

Abstract

Single Nucleotide Polymorphism on exon 2 of leptin gene which changes encoding from Arginine to Cysteine could change the function of Leptin as an obese gene which regulated feed intake and energy expenditure. The aims to analyze point mutation based on SNP exon 2 of Leptin gene of Pasundan cattle. This research used 49 DNA of Pasundan cattle from BPPT SP Cijeungjing. Multiplication of DNA was done by Polymerase Chain Reaction (PCR). Analyze of SNP on exon 2 Leptin gene based on nucleotide sequence from direct sequencing of PCR product which was alligned with leptin gene sequence from gene bank using Bioedit and MEGA 5.2 program. The results showed nucleotide sequence of exon 2 Leptin gene in Pasundan cattle is different from Leptin gene sequence of Bos indicus and Bos taurus. It was found one synonymous SNP that did not change amino acids Serine encoding on S17S, and two non-synonymous SNP which altered amino acids encoding, i.e R25C and R25H. In Pasundan cattle, the frequency of C allele (42.86%) was higher than A allele (31.64%) and T allele (25.50%). Six genotypes were identified i.e. CC (24.49%), CT (32.65%), CA (4.08%), TT (8.16%) and TA (2.05 %), AA (28.57%).