Abstract
Prolactin gene plays crucial role in the reproduction and egg production of birds. The objectives of this study were to characterize single nucleotide polymorphism in partial intron and coding region of duck prolactin gene. Blood samples were collected from 168 ducks consisted of 19 Pekin, 36 Mojosari, and 113 of their crossbreds collected from Indonesian Research Institute for Animal Production (IRIAP). Primer pairs for the coding regions in prolactin gene were self designed based on the duck genomic sequence database (GeneBank: AB158611.1). PCR products based on DNA of prolactin gene exon two was amplified approximately 400 bp. There is one base insertion of Adenin at the position of 2001 bp intron two region of duck prolactin. Homology test based on BLAST method indicated 99% identity with duck refference (Code Access GeneBank: AB158611.1). Adenin composition in all of duck samples was higher than refference. Triplet hydrogen bonds between Guanine and Cytosin pairs was higher than those at duplet hydrogen bonds between Adenine and Thymine. All duck samples were homozigous and monomorphyc.
Highlights
Indonesia has many species of local duck such as Mojosari from East Java, Alabio from Kalimantan, Bali duck, Tegal, Cirebon, Magelang, Kerinci, Cihateup, Turi, Pitalah, Talang Benih, Bayang, Rambon and Pegagan
Blood samples were collected from 168 ducks consisted of 19 Pekin, 36 Mojosari, and 113 of their crossbreds collected from Indonesian Research Institute for Animal Production (IRIAP)
Homology test based on Basic Local Allignment Search Tool (BLAST) method indicated 99% identity with duck refference (Code Access GeneBank: AB158611.1)
Summary
Indonesia has many species of local duck such as Mojosari from East Java, Alabio from Kalimantan, Bali duck, Tegal, Cirebon, Magelang, Kerinci, Cihateup, Turi, Pitalah, Talang Benih, Bayang, Rambon and Pegagan. Genetic approach to cross Pekin and Mojosari ducks for producing Pekin Mojosari crossbred is one of the method to improve meat performance of local duck. Prolactin is a single chain polypeptide protein secreted in the cephalic lobe of the anterior pituitary gland and acts on peripheral target tissues as hormone (Kansaku et al 2005). It is coded by prolactin gene and involved in a broad variety of biological functions in all vertebrates (Forsyth & Wallis 2002). The objectives of this study was to characterize single nucleotide polymorphism of prolactin gene exon two and partial intron in ducks of. Primer designed based on duck prolactin gene (Code Access AB158611.1) using primer (Table 2)
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