Single nucleotide polymorphism of IL-18 gene and resistin gene in children infection with Entamoeba histolytica

Abstract

Abstract Background: Human amebiasis is caused by Entamoeba histolytica, which is found in many tropical countries. E. histolytica infections are known to have a wide range of clinical consequences. Most infections are asymptomatic; some cause diarrhea and dysentery, and only a few cause extraintestinal consequences, including liver abscess. Single nucleotide polymorphisms (SNPs) are the most prevalent kind of sequence variation in genomes and are regarded as useful genetic markers for exposing the evolutionary history and common genetic variants that explain the heritable risk for common illnesses and effected in the progression of some infections. Objective: Investigation of gene polymorphism of the parameters (interleukin 18 [IL-18], resistin) most susceptible to parasitic infection. Materials and Methods: Stool samples were collected from patients who were attending the (Kerbala Teaching Hospital for children in the holy city of Kerbala), between February 2021 and January 2022. In total, 3748 samples of feces from children between the ages of 1 and 15 years from both gender were tested using both direct smear and acid-fast stain tests in addition to rapid test techniques. Five milliliters of blood were obtained from 25 patients infected with E. histolytica, as well as 5 mL of blood from 25 healthy youngsters (sample control). Results: The result showed the distribution of genotype of IL-18 SNPs in intestinal parasites patient in contrast to the control group; there is an increase in the level of IL-18 in some SNPs, resistin concentration was statistically significantly different between resistin gene polymorphisms. Conclusion: The IL-18 and resistin polymorphism can be considered one of the genetic factors responsible for the progression of E. histolytica infection.