Abstract
Biological nanopores are currently being investigated as a fast, low cost DNA sequencing platform. Single stranded DNA (ssDNA) is electrophoretically driven through a protein pore as the ionic current through the constriction is measured. The porin MspA of Mycobacterium smegmatis was mutated to produce a channel highly suitable for nanopore DNA sequencing. To study the resolution of the mutated porin MspA, we immobilize ssDNA within the pore using a streptavidin ‘anchor’. Each base, adenine, cytosine, thymine, and guanine, produces a distinct current signature when it is held within the nanopore.
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