Abstract
Single Mouse Oocyte Encapsulated in Medium-in-Oil Microdroplets by Using a Polydimethylsiloxane Microfluidic Device
Highlights
With structure design and sensor integration, micro-electromechanical systems (MEMS)-based devices have been widely used in chemical or biomedical research such as in chemical separation,(1) chemical detection,(2,3) protein crystallization,(4) single-nucleotide polymorphism (SNP) detection,(5) DNA extraction,(6,7) polymerase chain reaction,(8) cell culture,(9) cell separation,(10–12) and drug delivery systems.[13]
By using both DI water and human tubal fluid (HTF) medium for the medium inlet, they all showed that the volumes of microdroplets created by a slow flow rate (50 μl/h) are smaller than those created by a fast flow rate (500 μl/h) under the same flow rate of the oil phase
We found out that the volume of a DI water microdroplet was about twice larger than that of an HTF medium microdroplet generated at the same flow rate
Summary
With structure design and sensor integration, micro-electromechanical systems (MEMS)-based devices have been widely used in chemical or biomedical research such as in chemical separation,(1) chemical detection,(2,3) protein crystallization,(4) single-nucleotide polymorphism (SNP) detection,(5) DNA extraction,(6,7) polymerase chain reaction,(8) cell culture,(9) cell separation,(10–12) and drug delivery systems.[13]. To solve these problems, droplet-based microfluidics[9,14,15,16] was used in several biomedical applications such as single-cell hybridoma screening,(17) digital analysis of bacterial samples,(18) and immunoassay-based single-cell analysis and sorting.[19] Some research groups have used cross-flow or T-junction[20] structures for water-in-oil microdroplet dispersion, and others used co-flow microfluidics[21] for liquid emulsification. Recent IVF studies tend to use a continuous polydimethylsiloxane (PDMS) microfluidic device for biomedical sample processing including oocyte pretreatment, sperm sorting, and further embryo culturing.[12,22,23] it is hard to manipulate multiple oocytes in a continuous channel, and contamination is a serious problem in biomedical experiments. The volume of microdroplets can be controlled by changing the flow rates of syringe pumps
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