Abstract
Cel7a is a cellobiohydrolase that degrades cellulose and release the disaccharide cellobiose. To study the product-inhibition mechanism of the Cel7a from Trichoderma reesei, we developed a multimodal microscope and investigated the interaction of Cel7a with immobilized cellulose in the presence of cellobiose. Our custom-built microscope can image immobilized cellulose by interference reflection microscopy and localize binding of quantum dot-labeled Cel7a enzymes by TIRF with a precision of 2.8 nm at 100 frames/s.
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