Abstract
We developed a single-molecule four-color FRET technique both in confocal and in total-internal-reflection fluorescence microscopies. Real-time determination of six inter-fluorophore FRET efficiencies allowed us to probe the correlated motion of four arms of the Holliday junction. The technique was also applied to assess the correlation of RecA-mediated strand exchange events at both ends of a synaptic complex.
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