Abstract
Site-specific DNA recombination plays a key role in bacterial cell division, viral life cycles and recombinant DNA technology. We present a general method for observing the action of recombinase proteins on surface-immobilised DNA molecules using single-molecule fluorescence. In conjunction with FRET we use a standard TIRF microscope with alternating laser excitation to monitor the intensity and point-spread-function width of the FRET acceptor, enabling us to interrogate the diffusive freedom of the fluorophore, which in turn reports on large scale conformational changes of DNA.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
