Abstract
A single liposome fusion assay has been developed in our lab to unambiguously detect different stages of fusion including docking, hemi and full fusion via fluorescence resonance energy transfer. [1, 2] While this assay provides the extent of lipid mixing, the presence of the fusion pore opening cannot be detected. In order to further push the technique and to reveal the minimal machinery of SNARE-mediated membrane fusion, we have developed a single molecule assay based on content mixing of a large DNA probe.
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