Abstract
We report on a novel magnetic tweezers that can apply stresses ranging from 0.01 to 100 pN to single DNA molecules in the horizontal plane, allowing us to explore the functional mechanisms of DNA compacting proteins. We calibrated our instrument to the overstretching transition. We also performed calibration by comparing the measured bead motion to a model of Brownian motion in a harmonic potential well. We obtained estimates of the probe drag coefficient (α) and stiffness of the system (κ) using Allan variance (AV), power spectral density (PSD) methods, and compared those data to the calibration based on the fluctuation-dissipation method.
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