Abstract
In this review, single-chain fragment variable construction using phage-display technology as a promising anticancer immunotherapy technology is described. Cloning and the specific bio-panning selection with phage display technology, as well as the use of the epidermal growth factor receptor (EGFR) at the surface of MCF-7 cells as the antigen for the straightforward specific selection of single chain Fvs, are discussed. Moreover, phage display technologies and their application are important for vaccine production and immunotherapy against viruses and cancers. Furthermore, expression of the gene will cause the production and expression of the protein in prokaryotic and eukaryotic cells, which can be used to detect anti-cancer single chain fragment variables (scFvs). Finally, homology modelling is described to show the three-dimensional scFv structure that verifies the Complementary-Determining-Regions (CDRs) on the surface of the model.
Highlights
Breast cancer is the second major reason of death in women in age between 40 and 55 years after lung-cancer
Phage display technologies and their application are important for vaccine production and immunotherapy against viruses and cancers
The single chain fragment variables (scFvs)-MCF-7 products are highly recommended to be used in diagnosis and gene therapy toward breast cancer cell line MCF-7
Summary
Breast cancer is the second major reason of death in women in age between 40 and 55 years after lung-cancer. Sum of gene therapy methods for breast cancer have been advanced These methods can be alienated into the following six mean classes: 1) genetic assimilation therapy; 2) chemo-therapy; 3) disintegrate cell therapy; 4) gene therapy to reduce the growth of new blood vessels tumors that need to grow; 5) genetic immune-resistance; and 6) genetic resistance therapy. The use of IgG monoclonal antibodies is first start antibodies produced in hybridoma technology Those antibody targeted EGFR on the surface of MCF-7 breast cancer cells, which is function in to gene therapy and diagnosis of MCF-7 cancer cell. Which are constructed using phage display technology and molecular cloning and the specific bio-panning selection with the use of the epidermal growth factor receptor (EGFR) at the surface of MCF-7 cells as the antigen for the straightforward specific selection are considered as fast and accurate way of producing specific antibodies [3]. The scFv-MCF-7 products are highly recommended to be used in diagnosis and gene therapy toward breast cancer cell line MCF-7
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