Abstract
Single cell profiling was performed to assess differences in RNA accumulation in neighboring hyphae of the fungus Aspergillus niger. A protocol was developed to isolate and amplify RNA from single hyphae or parts thereof. Microarray analysis resulted in a present call for 4 to 7% of the A. niger genes, of which 12% showed heterogeneous RNA levels. These genes belonged to a wide range of gene categories.
Highlights
Cellular heterogeneity within an isogenic cell population is a widespread event in both prokaryotic and eukaryotic organisms
Distribution of nuclei and septa was monitored at the periphery of 7-day-old sandwiched colonies of A. niger using a fusion of the histone H2B protein and green fluorescent protein (H2B-GFP fusion) and calcofluor white, respectively
It is shown that hyphae that experience identical environmental conditions are heterogeneous with respect to RNA composition
Summary
Cellular heterogeneity within an isogenic cell population is a widespread event in both prokaryotic and eukaryotic organisms. Many documented cases of phenotypic variability in microorganisms relate to responses to environmental stress This suggests that phenotypic variation aids in the survival of cells under adverse conditions and may be an evolvable trait [1,2]. Protein secretion [3,4,5] and gene expression [6,7,8,9] are heterogeneous between zones of fungal colonies. These differences were explained by the availability of carbon source and by spatial and temporal differentiation [7]
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