Abstract
In mammals, the development of pluripotency and specification of primordial germ cells (PGCs) have been studied predominantly using mice as a model organism. However, divergences among mammalian species for such processes have begun to be recognized. Between humans and mice, pre-implantation development appears relatively similar, but the manner and morphology of post-implantation development are significantly different. Nevertheless, the embryogenesis just after implantation in primates, including the specification of PGCs, has been unexplored due to the difficulties in analyzing the embryos at relevant developmental stages. Here, we present a comprehensive single-cell transcriptome dataset of pre- and early post-implantation embryo cells, PGCs and embryonic stem cells (ESCs) of cynomolgus monkeys as a model of higher primates. The identities of each transcriptome were also validated rigorously by other way such as immunofluorescent analysis. The information reported here will serve as a foundation for our understanding of a wide range of processes in the developmental biology of primates, including humans.
Highlights
Background & SummaryFor more than half a century, mice have been exploited as a representative model organism for mammalian development and physiology
Even within such a transient period, the EPI cells show dynamic changes in pluripotency from a so-called naive to a primed state[1], and both states in mice have been captured in vitro: the naïve state is replicated in embryonic stem cells (ESCs)/induced pluripotent stem cells, which have essentially the same status as the pre-implantation EPI in vivo[2], while the primed state is replicated in epiblast stem cells (EpiSCs), which are derived from the post-implantation EPI and are homologs of the EPI of the gastrula[3,4,5]
The sequence reads were acquired by SOLiD5500xl and were mapped onto the cynomolgus monkey genome, Macaca fascicularis 5.0 (MacFas5.0)
Summary
For more than half a century, mice have been exploited as a representative model organism for mammalian development and physiology. The epiblast (EPI) is made up of cells specified during the peri-implantation period of early embryogenesis and differentiates into three primary germ layers and the germ cell lineages; the EPI bears the pluripotency Even within such a transient period, the EPI cells show dynamic changes in pluripotency from a so-called naive to a primed state[1], and both states in mice have been captured in vitro: the naïve state is replicated in ESCs/induced pluripotent stem cells (iPSCs), which have essentially the same status as the pre-implantation EPI in vivo[2], while the primed state is replicated in epiblast stem cells (EpiSCs), which are derived from the post-implantation EPI and are homologs of the EPI of the gastrula[3,4,5]. The dataset in this Data Descriptor defined the first comprehensive molecular dynamics of primate early development, including early post-implantation embryogenesis, and will provide a foundation for future studies of primate development
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