Single-Cell RNA-Seq by Multiple Annealing and Tailing-Based Quantitative Single-Cell RNA-Seq (MATQ-Seq).

Abstract

Single-cell technologies have emerged as advanced tools to study various biological processes that demand the single cell resolution. To detect subtle heterogeneity in the transcriptome, high accuracy and sensitivity are still desired for single-cell RNA-seq. We describe here multiple annealing and dC-tailing-based quantitative single-cell RNA-seq (MATQ-seq) with ~90% capture efficiency. In addition, MATQ-seq is a total RNA assay allowing for detection of nonpolyadenylated transcripts.