Single cell RNA and immune repertoire profiling of COVID-19 patients reveal novel neutralizing antibody

Fang Li,Kexin Ma,Pingping Wang,Meng Luo,Wenyang Zhou,Chang Xu,Renqiang Liu,Zhaochun Xu,Jingwei Wang,Yuou Li,Qinghua Jiang,Xiyun Jin,Zheng Zhang,Zhigao Bu,Yiqun Li,Jinliang Li,Liran Juan,Huimin Cao

doi:10.1007/s13238-020-00807-6

Abstract

Single cell RNA and immune repertoire profiling of COVID-19 patients reveal novel neutralizing antibody

Highlights

Early-stage recovery patients maintain various immune responses and possess abundant protective antibodies in the circulation (Thevarajan et al, 2020)
To reveal the changes of immune cells caused by SARSCoV-2 infection, 8 healthy controls with single cell transcriptome data profiled by 10x Genomics were added in this study (Table S2 and Supplementary Materials)
PBMC samples from recovered COVID-19 patients at discharge were collected and simultaneously performed single cell RNA-seq with 5′VDJ capture and deep B cell repertoire sequencing. (B) UMAP map of B cells from twelve COVID-19 patients and eight healthy controls, which formed a gradient of transcriptional states from naïve B cells to an activated memory B cells to plasma cells. (C) Barplot showing the percentages for different B cell subgroups identified from single cell analysis, including naïve B cell (C10), resting memory B cells (C16), activated B cells (C24) and plasma cells (C27)

Summary

Introduction

Early-stage recovery patients maintain various immune responses and possess abundant protective antibodies in the circulation (Thevarajan et al, 2020). Sequencing (scRNA-seq), single cell BCR sequencing (scBCR-seq) and deep BCR repertoire profiling to prioritize the therapeutically relevant neutralizing antibody sequences in patients who have recently cleared the virus. To reveal the changes of immune cells caused by SARSCoV-2 infection, 8 healthy controls with single cell transcriptome data profiled by 10x Genomics were added in this study (Table S2 and Supplementary Materials).

Results

Conclusion