Abstract
Advances in single-cell RNA sequencing technologies and bioinformatics methods allow for both the identification of cell types in a complex tissue and the large-scale gene expression profiling of various cell types in a mixture. In this report, we analyzed a single-cell RNA sequencing (scRNA-seq) dataset for the intact adult mouse sciatic nerve and examined cell-type specific transcription factor expression and activity during peripheral nerve homeostasis. In total, we identified 238 transcription factors expressed in nine different cell types of intact mouse sciatic nerve. Vascular smooth muscle cells have the lowest number of transcription factors expressed with 17 transcription factors identified. Myelinating Schwann cells (mSCs) have the highest number of transcription factors expressed, with 61 transcription factors identified. We created a cell-type specific expression map for the identified 238 transcription factors. Our results not only provide valuable information about the expression pattern of transcription factors in different cell types of adult peripheral nerves but also facilitate future studies to understand the function of key transcription factors in the peripheral nerve homeostasis and disease.
Highlights
The human nervous system is comprised of two parts: the central nervous system (CNS) and the peripheral nervous system (PNS)
We identified 238 Transcription factors (TFs) expressed in intact mouse sciatic nerves and mapped their cell-type specific expression
Clustering of cells was based on gene expression using Seurat-identified clusters of Myelinating Schwann cells (mSCs) and nonmyelinating Schwann cells (nmSCs), epineurial, perineurial, and endoneurial fibroblasts, endothelial cells (ECs), vascular smooth muscle (VSM) cells, pericytes, and immune cells (Figures 1A,B)
Summary
The human nervous system is comprised of two parts: the central nervous system (CNS) and the peripheral nervous system (PNS). The PNS consists of the nerves and the ganglia outside the brain and the spinal cord. The PNS is divided into the somatic nervous system and the autonomic nervous system. Peripheral Nerve Transcription Factors system transmits signals from the brain to organs and striated muscles and is associated with the voluntary control of body movements via skeletal muscles. The somatic nervous system includes the sensory nervous system that transmits signals from senses, such as taste, touch, and pain to the spinal cord and the brain. The autonomic nervous system acts largely unconsciously to regulate the physiological functions of the internal organs, such as the heart rate, digestion, respiratory rate, pupillary response, urination, and sexual arousal
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