Abstract
Aims: Embryo sexing is one of the important ways for sex selection of offspring. This is a potential method to considerably improve animal breeding and the efficiency of dairy and meat production. A novel repeated sequence specific to male cattle ha s been identified and named S4. S4 is a 1.5 Kb repeating unit contains various internal repeated sequence. S4 is localized on long arm of the Y chromosome in the region near to ZFY genes. The objective of this s tudy was to establish a simple, sensitive, reliable, reproducible and cost effective PCR based technique for sexing. Study Design : Case control study.
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