Single Cell Oil Production by Wild Type Strain Lipomyces starkeyi Y604

Abstract

As one of the promising oleaginous yeast, Lipomyces starkeyi has emerged as a great candidate of a single cell oil (SCO) producer. This is due to its ability to accumulate high lipid content and its ability to consume a diverse carbon sources. However, lipid production of the wild type strain is limited and efforts to obtain high producing wild-type strain are demanded. For this purpose, we conducted fermentation of the local wild-type strain of L. starkeyi InaCC Y604, derived from the collections of Indonesian Culture Collection (InaCC). In this study, we employed glucose, xylose, and a combination of glucose and xylose as carbon sources and conducted the fermentation in the batch flask scale. Following the fermentation, parameters such as dry cell weight (DCW), carbon source consumption, SCO production, lipid content and lipid yield were determined. In this study, we obtained that L. starkeyi InaCC Y604 grown in medium contained a mixture of glucose (50 g/L) and xylose (50 g/L) accumulate highest SCO production with 57.04±0.94 g/L and its lipid content reached out 0.17±0.00 w/w. Despite the result is not the best among previous reported literatures, this shows that the Indonesian local strain L. starkeyi InaCC Y604 can be a promising candidate to be further optimized.