Abstract
Efforts have been taken to enhance the study of single‐cells, however, the task remains challenging because most previous investigations cannot exclude the interactions between single cells or separately retrieved cells with specificity for further analyses. Here, a single‐cell mobility analysis platform (SCM‐Chip) is developed that can not only real‐time monitor single‐cell migration in independent niches but can also selectively recover target cells one by one. The design of each channel with a single‐cell capture unit and an outlet enables the system to place single cells in different isolated niches with fluidic capture and to respectively collect target cells based on mobilities. SCM‐Chip characterization of breast cancer cells reveals the presence of high‐ and low‐migratory populations. Whole‐cell transcriptome analysis establishes that monocyte chemotactic protein induced protein 1 (MCPIP1) is related with cell mobility; cells with a high expression of MCPIP1 exhibit low mobility in vitro and metastasis in vivo. The SCM platform provides a generic tool for accurate single‐cell isolation and differentiation that can be readily adapted for the study of cancer and drug development.
Highlights
Efforts have been taken to enhance the study of single-cells, the task cancer invasion and metastasis;[2] it provides no information about the remains challenging because most previous investigations cannot exclude the migratory mechanisms of single cells
Evidence is mounting that metastatic cells exhibit increased genetic instability when compared with nonmetastatic cells, and such heterogeneity results in a difference in cell movement and channel with a single-cell capture unit and an outlet enables the system to place diversity in the expression of multiple single cells in different isolated niches with fluidic capture and to respectively collect target cells based on mobilities
Whole-cell transcriptome analysis establishes that monocyte chemotactic protein induced protein 1 (MCPIP1) is related with cell mobility; cells with a high expression genes.[3–5]
Summary
Efforts have been taken to enhance the study of single-cells, the task cancer invasion and metastasis;[2] it provides no information about the remains challenging because most previous investigations cannot exclude the migratory mechanisms of single cells. Whole-cell transcriptome analysis establishes that monocyte chemotactic protein induced protein 1 (MCPIP1) is related with cell mobility; cells with a high expression genes.[3–5] The study of cell migration at the single-cell level is important for the understanding of this complex process and heterogeneous biological system. After single-cell mobility array, a small number of breast cancer cells with different migratory capacities were retrieved from the SCM-Chip, followed by single-cell RNA sequencing for high-migratory cells versus low-migratory cells, which indicated a series of differ-
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