Single-cell manipulation on microfluidic chip by dielectrophoretic actuation and impedance detection

Abstract

This paper presents the design, fabrication, and characterization of a microfluidic biochip with integrated actuation electrodes used to manipulate a cell and a microbead by dielectrophoresis and sensing electrodes to detect the trapping by using the impedance detection method. Combining deflective dielectrophoretic barriers with controlled pressure-driven liquid flows allows the accurate control of a cell/microbead in suspensions. The threshold voltage for microbead trapping was experimentally verified at various flow rates. The impedance change caused by the blockage of the electrical conducting path between sensing electrodes with the trapping of an MCF7 cell and a polystyrene microbead was measured. The impedance before the trapping of an MCF7 cell was 10.9 MΩ at 1 kHz and increased to 12 MΩ when the cell was placed between sensing electrodes.