Abstract
This work describes a microfluidic system for in situ extraction of a single-cell and its phosphatidylcholine analysis through mass spectrometry. This approach uncovered cellular heterogeneity among seemingly identical cells and provided a new platform for identification and classification of cells.
Highlights
All publication charges for this article have been paid for by the Royal Society of Chemistry
We have developed a micro uidic-based in situ single-cell recognition system (ISCRS, Fig. S1, Electronic supplementary information (ESI)†) to extract a single-adhered-cell and analyze its phosphatidylcholine (PC) compositions through Mass spectrometry (MS)
There was little contact ratio between every group and the accuracy rate of classi cation was 91.8%. These results suggest the competency of this classi cation method for an effective identi cation of a single cell
Summary
All publication charges for this article have been paid for by the Royal Society of Chemistry. This work describes a microfluidic system for in situ extraction of a single-cell and its phosphatidylcholine analysis through mass spectrometry. This approach uncovered cellular heterogeneity among seemingly identical cells and provided a new platform for identification and classification of cells. We have developed a micro uidic-based in situ single-cell recognition system (ISCRS, Fig. S1, ESI†) to extract a single-adhered-cell and analyze its phosphatidylcholine (PC) compositions through MS. This system performed well for the isolation of a whole cell from culture medium which excludes the signal interference of the solution composition.
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