Abstract
Aim: Circulating tumor cells (CTCs) in the blood of ovarian cancer patients (OCP) are associated with decreased overall survival, whereby CTCs with epithelial-mesenchymal-transition (EMT) or stem-like traits are supposed to be involved in metastatic progression and recurrence. Thus, investigating the transcriptional profiles of CTCs might help to identify therapy resistant tumor cells and to overcome treatment failure. To this end, we established a multi-marker RT-PCR for the molecular characterization of single CTCs, detecting epithelial (EpCAM, Muc1, CK5/7), EMT (N-cad, vim, Snai1/2, CD117, CD146, CD49f) and stem cell (CD44, ALDH1A1, Nanog, SOX2, Notch1/4, Oct4, Lin28) associated transcripts.
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