Abstract
Similar to many nonexcitable cells, microglia utilize intracellular Ca2+ signaling for the communication with each other as well as neurons and astrocytes and for triggering a magnitude of their executive functions. However, in vivo measurements of the intracellular Ca2+ dynamics in microglia have been challenging due to technical reasons. Here, we describe an approach utilizing a single-cell electroporation technique to facilitate the study of microglial Ca2+ signaling in the living brain.
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