Single cell activity in ventral prefrontal cortex of behaving monkeys

Abstract

Single unit activity was recorded extracellularly from ventral prefrontal cortex (VPC) of monkeys during performance of two short-term memory tasks: spatial delayed response and delayed matching to sample. The tasks required perception, retention and recognition of visual cues differing in either color or spatial location. Two separate areas of VPC were explored: a lateral area in the lower prefrontal convexity and a medial area around the medial orbital sulcus. Two categories of unit activity were distinguished on the basis of frequency changes to the cue. One was characterized by non-specific discharge independent upon which cue was presented, the other by discriminative discharge related not only to visual qualities of the cue but to the animal's subsequent use of it. Nearly one-half of all units showed altered firing during the retention (delay) period as compared with intertrial control firing. Eighteen per cent displayed delay activity related to the quality of the preceding cue. The lateral and medial segments of VPC were not distinguished by differences of unit activity in cue or delay periods. Post-trial activity was related to presence or absence of reward. Type I cells showed firing changes following choice reinforcement as well as gratuitous reward; some showed changes in opposite direction following unreinforced choices. They may encode the availability of reward. Type II cells showed changes of activity after unreinforced trials and, in some cases, opposite changes after unexpected reward; they were not affected by the reward of normal correct-choice trials. These cells appear to react to deviations from expectancy of reward. Type III cells exhibited comparable firing changes following reinforced and unreinforced choices. They may encode termination of a trial sequence. Type I was more common in lateral than medial VPC, whereas the opposite was true for type II; type III did not clearly predominate in either area. Ablation studies have shown that the two areas of VPC differe in behavioral functions. This study of their cellular properties revealed topographic differences only during the post-trial period. It is therefore possible that the combination of cue and delay activity (related to exteroceptive input) with post-trial activity (related to interoceptive input) constitutes the neuronal basis for the two areas' differences in behavioral function.