Abstract
Background Quantitative measurement of basophil cell surface markers, CD63 and CD203c, by flow cytometry has been developed as useful tool for the diagnosis of IgE mediated allergies. Available methods require large volume of blood and include extra steps such as prior cell purification, priming with IL-3, stopping cell activation and fixing. We have developed a new basophil based test which can be performed with small amount of whole blood directly from skin prick or from small amount of the whole blood where multiple allergens can be tested at the same time for high-throughput testing.
Highlights
Quantitative measurement of basophil cell surface markers, CD63 and CD203c, by flow cytometry has been developed as useful tool for the diagnosis of IgE mediated allergies
We have developed a new basophil based test which can be performed with small amount of whole blood directly from skin prick or from small amount of the whole blood where multiple allergens can be tested at the same time for high-throughput testing
Control allergens did not show any change in the mean fluorescence intensity (MFI) before and after the incubation, which suggested allergen specific CD203c induction on basophil cell surface
Summary
Quantitative measurement of basophil cell surface markers, CD63 and CD203c, by flow cytometry has been developed as useful tool for the diagnosis of IgE mediated allergies. Single blood drop diagnostic activation test (DAT) for food allergies From Food Allergy and Anaphylaxis Meeting 2011 Venice, Italy. Background Quantitative measurement of basophil cell surface markers, CD63 and CD203c, by flow cytometry has been developed as useful tool for the diagnosis of IgE mediated allergies.
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