Single and mixture administration of white tea (Camellia sinensis) and moringa (Moringa oleifera) as antihyperglicemia in diabetic male rats (Sprague-dawley) induced by streptozotocin

Abstract

Hyperglycemia can increase oxidative stress by increasing the formation of reactive oxygen species (ROS) and alter redox reactions. Excessive ROS production leads to oxidative stress that can increase the product of lipid peroxidation. ROS production can be measured indirectly by measuring lipid peroxidation metabolites such as malondialdehyde (MDA) and F2-Isoprostan. The aim of this study was to determine the effect of single and mixture of white tea and moringa on oxidative stress markers (MDA and F2-Isoprostan) on streptozotocin-induced diabetic rats and compared with tea controls (green tea). Male Sprague-dawley rats, consist of 30 rats, were divided into six groups. Normalgroup was healthy rats without treatment, negative control group (NC) was streptozotocin-induced diabetic group without treatment, white tea treatment group (WT), moringa treatment group (M), mixture white tea and moringa treatment group (WT+M). The treatment were daily administrated by orally with 100 mg/kg BW epigallocatechin gallate (EGCG) for 21 days. MDA was measured by UV-VIS spectrophotometer at 535 nm and F2-Isprostane level was measured by ELISA. The Change of MDA levels (Δ MDA) between treatment groups showed that WT, M and WT+M were significantly different with NC group (p <0.05). Compared to thecontrol tea group (GT) showed that MDA levels of WT or M groups were different with Δ MDA GT group (p<0.05). WT+M group that decreased MDA levels did not differ significantly with GT (p>0.05). Change of F2-Isoprostan level between treatment groups (Δ F2-Isoprostan) showed that there were no significantly different to NC group (p> 0.05). It can be concluded that administration of white tea, moringa dan mixture of white tea+moringa may prevent or reduce MDA level in streptozotocin diabetic rats but not in F2-Isoprostane level. The combination of white tea+moringa (WT+M) has a better ability to decrease MDA level compared with single treatment and not significantly different with the control tea group (green tea).