Single and combined exposures of waterborne Cu and Cd induced oxidative stress responses and tissue injury in female rare minnow (Gobiocypris rarus)

Abstract

Copper (Cu) and cadmium (Cd) are two kinds of abundant toxic metals in aquatic ecosystem. The present study evaluated the effects of waterborne Cu and Cd on oxidative stress responses and histological alterations in female rare minnow (Gobiocypris rarus). Fish were exposed for 7 days to: (i) control (no added Cu or Cd), (ii) waterborne Cu (39.2 μg L−1), (iii) waterborne Cd (299.6 μg L−1), and (iv) Cu and Cd in mixture (19.6 and 149.8 μg L−1, respectively). Antioxidant enzyme activities and gene mRNA abundance in fish tissues (gills, liver, and ovaries) were induced by Cu and Cd exposures, both individually and in mixture, at day 1, but an asynchronous response was observed between most enzyme activities and gene mRNA abundance following 7 days exposure. Biochemical analysis and histological observation indicated that exposure to Cu and Cd, alone and in combination, caused evident damage to lipids and tissue structure in gills, liver and ovaries. Comparing with single Cu or Cd exposure, Cu and Cd co-exposure induced greater increase in the mRNA expression of most antioxidant genes and caused more severe lesions in fish tissues, which suggested that exposure to waterborne Cu and Cd in mixture might increase their individual toxicity. Furthermore, positive relationships between nuclear factor erythroid 2-related factor (Nrf2) expression and expression of manganese superoxide dismutase (Mn-SOD) and catalase (CAT) were also observed in the present study, which suggested that Cu or/and Cd induced expression of these antioxidant genes were might through activation of Nrf2.