Single and combined effects of heat and water stress and recovery on cotton (Gossypium hirsutum L.) leaf physiology and sucrose metabolism

Abstract

High temperatures and water-deficit stress limit cotton production around the world. Their individual effects on plant physiology and metabolism have been extensively studied, however, their combination has received considerably less attention. To that end, growth chamber experiments were conducted using cotton (Gossypium hirsutum L.) cultivar ST5288B2F and the objectives were to discern physiological and metabolic alterations after heat and water stress application (single or combined) and recovery, during cotton's vegetative stage. Under water stress conditions, leaf physiological parameters were suppressed and changes in carbohydrate levels, due to alterations in sucrose-metabolizing enzymes activities, were observed. Heat stress alone increased carbohydrate content, and activities of sucrose-degrading enzymes, while leaf physiology remained unaffected. The combined stress did exacerbate decreases in leaf water potential and soluble acid invertase activity, but the rest of the responses were similar to those of water stress. After stress alleviation, leaf physiological parameters of water-stressed plants did not manage to recover and substantial decreases were observed in leaf starch levels and activities of sucrose-cleaving enzymes, while the majority of parameters of heat-shocked plants returned to control levels. Recovery of the plants subjected to the combined stress was comparable to that of water-stressed plants, but significant differences were observed in carbohydrate levels and sucrose synthase activity. Our study demonstrated that under combined stress and post-stress conditions, water stress was the dominant factor affecting cotton leaf physiology and sucrose metabolism, highlighting however, the unique responses of some traits that could not be deduced from the additive effects of the single stresses.