Simultaneously increased expression of the c‐myc and, mU chain genes in the acute blastic transformation of a chronic lymphocytic leukaemia

Abstract

A B-cell chronic lymphocytic leukaemia terminated, 5 years from the onset, with a blast crisis. Karyotype analysis showed that the terminal lymphoblastic population evolved from the original B lymphocytic clone. The levels of expression of several oncogenes, as well as the mu chain gene, were assayed by Northern blot hybridization analysis of RNA extracted from the lymphoid populations before and after the 'acute transformation'. A seven- to eight-fold increase in the expression of c-myc and mu chain genes was observed in the blast population. c-myb, c-fes, c-Haras were not expressed either before or after the blastic transformation.