Simultaneously attenuating antibiotic resistance genes and improving the dewaterability of sewage sludge by conditioning with Fenton's reagent: the pivotal role of sludge pre-acidification.

Abstract

Fenton conditioning processes have been recently employed to improve the dewaterability of sewage sludge. However, it remains unclear whether the conditioning with Fenton's reagent would simultaneously attenuate antibiotic resistance genes (ARGs) in sludge and improve sludge dewaterability. It was found in the present study that sludge pre-acidification played a pivotal role in simultaneously removing ARGs and improving sludge dewaterability by conditioning with Fenton's reagent. When the sewage sludge was pre-acidified to pH = 3.0 and was then conditioned using Fenton's reagent, the absolute abundances of the total ARGs and the total mobile genic elements (MGEs) in conditioned sludge were reduced by 1.85-2.10 and 2.84-3.12 log units, respectively. Additionally, sludge capillary suction time (CST) and specific resistance to filtration (SRF) were drastically reduced, and the moisture content (MC) in dewatered sludge cake was reduced to only 60.61-69.95%. Such effective attenuation of ARGs and MGEs in conditioned sludge led to their removal in both the dewatered sludge cakes and dewatering filtrate. However, only the improvement of sludge dewaterability was attained by sludge conditioning with Fenton's reagent but without sludge pre-acidification. During the conditioning treatment, the removal of loosely bound extracellular polymeric substance (EPS) and tightly bound EPS in conditioned sludge contributed to the improvement of sludge dewaterability, and the damage of sludge microbial cells was highly correlated with the attenuation of antibiotic resistance. Thus, sludge pre-acidification combined with conditioning using Fenton's reagent can be employed to simultaneously attenuate the antibiotic resistance in sewage sludge and improve sludge dewaterability.