Abstract

An automatic direct spectrophotometric method for the simultaneous determination of nitrite and nitrate by flow-injection analysis has been developed. The method is based on the reaction of nitrite and nitrate with the spectrophotometric reagent N-phenylanthranilic acid in sulfuric acid medium (pH=0.4∼0.6) to form the same color products while the absorbance is measured at 410 nm. This method has the advantage of direct determination of nitrite and nitrate and without reducing nitrate to nitrite as other reported methods. Various analytical parameters, such as flow rate, sample size, effect of acidity(pH), reagent concentration and interfering species were studied. The detection limit is 2.5 ng ml−1 for nitrite and 12 ng ml−1 for nitrate. Up to 35 samples per hour can be analyzed with a relative precision of ca. 0.1–2%.

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call

Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.