Simultaneous Spectrophotometric Determination of Group B Vitamins Using Parallel Factor Analysis: PARAFAC

Abstract

In this work, a simple and rapid analytical procedure was applied for simultaneous determination of folic acid (vitamin B 0 ), thiamin (vitamin B 1 ), riboflavin (vitamin B 2 ) and pyridoxal (vitamin B 6 ) based on the absorbance data in the pH range 2.0-12.0 at 25 °C using parallel factor analysis (PARAFAC). The effect of the pH as the most important factor on the sensitivity of the determination was studied. The spectral data were recorded in 400-650 nm intervals and a 2-12 pH range for all four vitamins. The calibration set was constructed in the concentration ranges of 4-22, 1-20, 6-26, and 4-20 pg mL -1 for B 6 , B 2 , B 1 and B 0 , respectively. The root mean squares errors of prediction for the prediction set, (RMSEP), are 0.65, 0.63, 1.13 and 0.34 for B 0 , B 1 , B 2 and B 6 , respectively. The recovery percent for the validation set are in the range of 90.6 to 107.0%. The effect of the experimental conditions and diverse species were discussed. The optimum values of these factors were searched according to the relative standard deviation of the prediction set of mixtures solutions.