Abstract
We have developed a reversed‐phase C18 high performance liquid chromatography (HPLC) method to separate molecular species of monoacylglycerols (MAG), fatty acids, fatty acid methyl esters, and fatty acid ethyl esters, simultaneously. This system also separates the regioisomers, 2‐acyl‐sn‐glycerol and 1‐acyl‐sn‐glycerol, with 2‐acyl‐sn‐glycerol eluting earlier than 1‐acyl‐sn‐glycerol. The elution order of the fatty acid and esters was ricinoleate, linolenate, linoleate, palmitate, oleate, and stearate. This method is also useful for the identification of radiolabeled metabolites using co‐chromatography.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Journal of Liquid Chromatography & Related Technologies
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
