Abstract
Abstract A column chromatographic technique utilizing basic alumina at pH 4.5 for the separation of homovanillic acid (HVA) and subsequent differential elution of vanillylmandelic acid (VMA), dihydroxyphenylacetic acid (DOPAC) and dihydroxymandelic acid (DOMA) is presented. The 24-h urinary excretions of these acids (with separate day and night specimens) were measured in 12 normal adult males. Mean 24-h urinary outputs expressed in mg/24 h are 9.4 ± 2.5 for HVA, 3.6 ± 1.2 for VMA, 3.0 ± 1.3 for DOPAC and 0.34 ± 0.13 mg for DOMA. Except for an increased day excretion of DOPAC there was no significant difference in day and night output of these compounds. As little as 3 μg of an authentic mixture of DOMA and DOPAC could be separated on basic alumina, with respective recoveries of 57 and 53%. The same concentration of both compounds added to rat brain homogenate yielded recoveries of 55% for DOMA and 56% for DOPAC. The advantages and application of basic alumina for the separation of blood plasma dihydrophenylalanine (DOPA) from dopamine are discussed.
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