Simultaneous Separation and Analysis of Five Compounds in Cibotium barometz by Micellar Electrokinetic Chromatography with Large-Volume Sample Stacking

Lili Wang,Lishuang Yu,Xihai Li,Zaishi Zhu,Jun Peng,Huifeng Xu,Linglong Liu,Hongzhi Ye

doi:10.3390/separations8090147

Lili Wang, Lishuang Yu + Show 6 more

Open Access

https://doi.org/10.3390/separations8090147

Copy DOI

Abstract

A large volume sample stacking (LVSS) method in micellar electrokinetic chromatography (MEKC) with diode array detector was developed for the simultaneous separation and analysis of five compounds: protocatechuic acid, protocatechuic aldehyde, caffeic acid, syringetin and vanillin in Cibotium barometz. The electrophoretic separation was performed in a 10 mM sodium dodecyl sulfate (SDS) and 50 mM sodium borax-sodium dihydrogen phosphate system (pH = 8.5) with 10% methanol at a separation voltage of 30 kV after optimizing the typical parameters. The detection limits were from 32 pg to 65 pg, which were around 12–27 times lower than MEKC, and 500 times less than reported methods. Finally, the established method was validated to be applicable for the determination of protocatechuic acid and caffeic acid in Cibotium barometz. This proposed method is expected to facilitate the quality control of Cibotium barometz.

Highlights

As an important traditional Chinese medicine, Cibotium barometz is widely used to treat limb-ache, rheumatism, sciatica and osteoporosis [1]
Evidence shows that protocatechuic aldehyde, protocatechuic acid, caffeic acid and polysaccharides from rhizomes of Cibotium barometz had a significant proliferative effect on osteoblasts [9,10]
Protocatechuic acid, protocatechuic aldehyde, caffeic acid, syringetin and vanillin were obtained from Yuanye Biotech (Shanghai, China)

Summary

Introduction

As an important traditional Chinese medicine, Cibotium barometz is widely used to treat limb-ache, rheumatism, sciatica and osteoporosis [1]. It has the properties of being anti-inflammatory [2], reducing bone loss [3], increasing antioxidant activity [4,5] and promoting chondrocyte proliferation [6]. Cibotium barometz mainly contains protocatechuic acid [1,7] and protocatechuic aldehyde [1,7], in addition to caffeic acid [1,8], syringetin and vanillin [8]. Evidence shows that protocatechuic aldehyde, protocatechuic acid, caffeic acid and polysaccharides from rhizomes of Cibotium barometz had a significant proliferative effect on osteoblasts [9,10]. Protocatechuic acid and protocatechualdehyde were determined by HPLC [11,12]

Methods

Results

Conclusion