Simultaneous saccharification and fermentation of enzyme pretreated Lantana camara using S. cerevisiae

Abstract

Lantana camara, an abundantly available non-edible lignocellulosic biomass has been found to be a potential feedstock for ethanol production. The substrate was first pretreated with laccase followed by simultaneous saccharification and fermentation using cellulase and Saccharomyces cerevisiae, respectively. Laccase was produced from Pleurotus sp. and carbohydratases (cellulase and xylanase) were produced from Trichoderma reesei Rut C30. Using pretreated substrate simultaneous saccharification and fermentation was optimized through central composite design-based response surface methodology. Maximum bioethanol concentration of 5.14% (v/v) was obtained at optimum process conditions of substrate concentration 17% (w/v), inoculum volume 9% (v/v), inoculum age 60 and 144h of incubation time. To enhance ethanol yield, S. cerevisiae was treated with ethyl methane sulfonate, a chemical mutagenic agent which induced mutagenesis. A maximum bioethanol concentration of 6.01% (v/v) was obtained using the mutated strain of S. cerevisiae (CM5).