Abstract
A simple, precise, accurate, rapid and reproducible reverse phase high performance liquid chromatographic procedure was developed for simultaneous determination of nitazoxanide and ofloxacin in tablet dosage form at a single wavelength. The mobile phase used was a combination of acetonitrile:0.25M potassium dihydrogen phosphate buffer (80:20) with 0.5%v/v of triethylamine and the pH was adjusted to 2.5 by adding orthophosphoric acid. The detection of the combined dosage form was carried out at 320 nm and flow rate was set to 1ml/min. Linearity was obtained in the concentration range of 5 to 25 μg/ml of nitazoxanide and ofloxacin with correlation coefficients of 0.9987 and 0.9995, respectively. The results of the analysis were validated statistically and recovery studies confirmed the accuracy of the proposed method.
Highlights
A simple, precise, accurate, rapid and reproducible reverse phase high performance liquid chromatographic procedure was developed for simultaneous determination of nitazoxanide and ofloxacin in tablet dosage form at a single wavelength
Few UV spectrophotometric methods and HPLC methods have been reported for the estimation of ofloxacin, especially in body fluids and blood plasma as well as from pharmaceutical preparations[7,8,9,10,11]
Literature review revealed that no HPLC method is yet reported for the simultaneous estimation of the NTZ and OFX in combined tablet dosage form at single wavelength
Summary
7.17 5-25 99-102 200.03 0.14 0.43 1.37 0.55 3.38 28503 123909 0.9987 No interference >24 hours. 1 ml from that solution was taken and diluted with mobile phase to make up to 10 ml. With the help of pipette 0.5, 1.0, 1.5, 2.0, 2.5 ml of the Þltered solution taken in small test tubes and diluted up to 10 ml of each respectively which contain 5:2, 10:4, 15:6, 20:8, and 25:10 μg/ml of NTZ and OFX respectively. In the similar way 50 mg of OFX was dissolved in 10 ml of buffer and further diluted with 40 ml of mobile phase, and the concentration of 1 mg/ml was obtained. The mobile phase acetonitrile:0.25 M potassium dihydrogen phosphate buffer (80:20) with 0.5%v/v of triethylamine, adjusted to pH 2.5 using orthophosphoric acid was found to be satisfactory and gave two symmetrical and well resolved peaks for NTZ and OFX. The wavelength 320 nm was selected for simultaneous determination based on maximum absorption of both drugs and best detector response at this wavelength
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