Abstract
A new, simple, precise, rapid and accurate RP-HPLC method has been developed for the simultaneous estimation of cefpodoxime proxetil and clavulanic acid from pharmaceutical dosage forms. The method was carried out on a Zorbax Eclipse XDB 5 μ C 18 (150×4.6 mm) column with a mobile phase consisting of acetonitrile:50 mM potassium dihydrogen phosphate buffer (pH 3.0, 70:30 v/v) at a flow rate of 1.0 ml/min. Detection was carried out at 228 nm. Aspirin was used as an internal standard. The retention time of clavulanic acid, cefpodoxime proxetil and aspirin was 4.43, 6.44 and 5.6 min, respectively. The developed method was validated in terms of accuracy, precision, linearity, limit of detection, limit of quantification and solution stability. The proposed method can be used for the estimation of these drugs in combined dosage forms.
Highlights
The linearity of the method was determined at five concentration levels ranging from 70 to 350 μg/ml for cefpodoxime proxetil and 20 to 100 μg/ml for clavulanic acid
No marked changes in the chromatograms demonstrated that the HPLC method developed is rugged and robust
The results show that for both solutions, the retention time and peak area of cefpodoxime proxetil and clavulanic acid remained almost unchanged (% RSD is less than 2) with no significant degradation within the period, indicated that both solutions were stable for at least 5 h, which was sufficient to complete the whole analytical process
Summary
570 nm 15 min 8-18 μg/ml 0.17543 μg/cm2/0.001 absorbanc units 5.7 × 10 mole-1 cm-1. Table showing summary of analytical parameters for developed spectrophotometric method. The proposed method is simple, accurate, precise sensitive and can be successfully applied for routine quantitative estimation of metformin hydrochloride in bulk and solid dosage forms. The summary of the method developed is shown in the Table 3
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