Simultaneous RP HPLC Determination of Aceclofenac, Paracetamol and Tizanidine in Pharmaceutical Preparations

V V Vaidya,M P Choukekar,M B Kekare,G R Singh

doi:10.1155/2010/323410

Abstract

A simple, fast and precise reverse phase high performance liquid chromatographic method is developed for the simultaneous determination of aceclofenac, paracetamol and tizanidine. Chromatographic separation of the three drugs were performed on a hypersil C18column (250 mm x 4.6 mm, 5 µm) as stationary phase with a mobile phase comprising of mix phosphate buffer pH 7.0: acetonitrile (40:60 v/v), at a flow rate of 0.7 mL min-1and UV detection at 230 nm. The proposed method was validated for linearity, accuracy, precision, LOD, LOQ. Linearity, accuracy and precision were found to be acceptable over the ranges of 100-300 µg mL-1for aceclofenac, 500-1500 µg mL-1for paracetamol and 2-6 µg mL-1for tizanidine HCl equivalent to tizanidine. It can be conveniently adopted for routine quality control analysis.

Highlights

Aceclofenac {[2-(2', 6'-dichlorophenyl) amino] phenyl acetoxyacetic acid} is a new phenyl acetic acid derivative with potent analgesic and anti-inflammatory properties and improved gastric tolerance[1]
A typical HPLC chromatogram for simultaneous determination of aceclofenac, paracetamol and tizanidine from pharmaceutical formulation is shown in Figure 4 and Figure 5
The limit of detection (LOD) and limit of quantitation (LOQ) of aceclofenac, paracetamol and tizanidine were experimentally determined by six injections of each drug

Summary

Introduction

Aceclofenac {[2-(2', 6'-dichlorophenyl) amino] phenyl acetoxyacetic acid} is a new phenyl acetic acid derivative with potent analgesic and anti-inflammatory properties and improved gastric tolerance[1]. The literature revealed that, no method was available for simultaneous determination of these three drugs in such pharmaceutical preparations by HPLC. An HPLC method was developed for determination of aceclofenac, paracetamol and tizanidine from their combined dosage form[6,7,8,9,10,11]. The method described is simple, fast, precise and accurate for simultaneous determination of aceclofenac, paracetamol and tizanidine from pharmaceutical preparation.

Results

Conclusion