Simultaneous removal of NH4+ and SO42− in Sulfate-reducing anammox scale reactor using FDAARGOS_798 strain/Anammox integration

Abstract

In this study, an exploratory experiment conducted to assess the efficiency of time reducing Bacillus cereus FDAARGOS_798 strain in the Sulfate-reducing anaerobic ammonium oxidation –Sulfammox- process at lab-scale reactor. The sulfammox process was examine in four different semi-continuous stirred (SCS) reactors receiving a treated synthetic wastewaters by 1 % of FDAARGOS_798 strain acclimated and carried with 2 % anaerobic granular sludge (sludge) in the presence and absence of the 1 % anammox consortia granular sludge (Anammox). Subsequently, the previous work outcomes of optimized anaerobic acclimation method isolating FDAARGOS_798 strain. The SCS-2 reactor with sulfammox integration (FDAARGOS_798/Anammox) has shown significant performance in anoxic simultaneous removal of NH4 + and SO42-, 77 ± 0.2 % and 50 ± 1.0 %, respectively, with the sole substrate (NH4)2SO4 0.1 g/L. The NH4+ and SO42- average decrease of 40 ± 0.2 % and 46 ± 0.17 % in 26 days, respectively, in SCS-4 reactor treated by sulfammox (FDAARGOS_798/Anammox) in (NH4)2SO4 0.5 g/L. Subsequently, the N/S ratio is calculated at around 1.8 ± 0.2 as evidence of reaching sulfammox process. The sulfammox process could not detect at SCS-1and SCS-3 reactors which are in the absence of sulfammox integration. Meanwhile the exact sulfammox process pathway was not detectable due to complicated scenarios. In conclusion, the addition of Bacillus ceruse FDAARGOS_798 carried by Anammox consortium is a well indicate to an effective and economical way that accelerate the start-up of the sulfate-reducing anammox process.