Abstract
Quantum dots-labeled urea-enzyme antibody-based rapid immunochromatographic test strips have been developed as quantitative fluorescence point-of-care tests (POCTs) to detect helicobacter pylori. Presented in this study is a new test strip reader designed to run on tablet personal computers (PCs), which is portable for outdoor detection even without an alternating current (AC) power supply. A Wi-Fi module was integrated into the reader to improve its portability. Patient information was loaded by a barcode scanner, and an application designed to run on tablet PCs was developed to handle the acquired images. A vision algorithm called Kmeans was used for picture processing. Different concentrations of various human blood samples were tested to evaluate the stability and accuracy of the fabricated device. Results demonstrate that the reader can provide an easy, rapid, simultaneous, quantitative detection for helicobacter pylori. The proposed test strip reader has a lighter weight than existing detection readers, and it can run for long durations without an AC power supply, thus verifying that it possesses advantages for outdoor detection. Given its fast detection speed and high accuracy, the proposed reader combined with quantum dots-labeled test strips is suitable for POCTs and owns great potential in applications such as screening patients with infection of helicobacter pylori, etc. in near future.
Highlights
Gastric carcinoma is a malignant tumor that is highly common all over the world and ranks second among all malignant tumors in China [1]
Characterization of CdSe quantum dot (QD) The QDs were CdSe core/ZnS shell QDs with carboxy1 groups displayed on their surface, which formed 6-nm particles in diameter and had a good dispersion
TEM picture of the QDs is displayed in Fig. 5a, showing that the water-soluble QDs have a diameter of 6 nm
Summary
Gastric carcinoma is a malignant tumor that is highly common all over the world and ranks second among all malignant tumors in China [1]. The cure rate of earlystage gastric carcinoma reaches up to 85–90 %, whereas that of late stage is less than 24 %. The early detection of gastric carcinoma is paramount to reduce the risk of patient mortality. Zheng et al Nanoscale Research Letters (2016) 11:62 had been infected with helicobacter pylori. The initiation and development of gastric carcinomas are closely linked to helicobacter pylori, which are important source of high-activity urea enzymes in the stomach [2–4]. Urea-enzyme detection in patient serum samples by immunochromatographic assay can confirm early-stage gastric carcinoma
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