Abstract
High-performance liquid chromatography (HPLC) is routinely used to analyze monosaccharide composition. This is the first report describing the simultaneous determination of uronic acid, amino sugar, and neutral sugar in the polysaccharide from Angelica sinensis using pre-column derivatization. We were able to analyze the monosaccharide composition of acidic Angelica polysaccharides (AAPS) using this method. Ten different monosaccharides, namely d-mannose, d-glucosamine, l-rhamnose, d-galactosamine, d-glucuronic acid, d-galacturonic acid, d-glucose, d-galactose, l-arabinose, and l-fucose were made into pre-column derivatives to serve as the standard for AAPS analysis. Samples were separated on a Hypersil BDS C18 column (4.6 mm × 250 mm, i.d.) and detected by UV detection at 250 nm wavelength. The mobile phase was composed of aqueous ammonium acetate buffer (100 mM, pH 5.0), acetonitrile, and tetrahydrofuran in the ratio of 81:17:2. Analytes were performed at 25 °C with a flow rate of 1.0 ml/min. Our study found that AAPS is composed of d-mannose, d-glucosamine, l-rhamnose, d-galactosamine, d-glucuronic acid, d-galacturonic acid, and d-glucose in a molar ratio of 1.5:14.1:1.7:1.6:2.0:2.3:1.
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