Simultaneous quantification of triterpenoic acids by high performance liquid chromatography method in the extracts of gum resin of Boswellia serrata obtained by different extraction techniques.

Neha Sharma,D K Gupta,Samar Singh,Satya Paul,Naresh K Satti,Vikram Bhardwaj,Suresh Chandra,Mahendra K Verma,Sheikh Abid Ali

doi:10.1186/s13065-016-0194-8

Neha Sharma, D K Gupta + Show 7 more

Open Access

https://doi.org/10.1186/s13065-016-0194-8

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Abstract

BackgroundBoswellia serrata, also known as Indian frankincense is a commercially important medicinal plant which has been used for hundreds of years as an Ayurvedic medicine for the attempted treatment of arthritis. It contains naturally occurring triterpenoic acids, called as boswellic acids (BA’s).ResultsA highly reproducible High performance liquid chromatography-ultraviolet diode array detection (HPLC-UV-DAD) method was developed for the simultaneous determination and quantitative analysis of eight major triterpenoic acids in Boswellia serrata gum resin obtained by different extraction techniques. All the calibration curves exhibited good linear regression (R2 > 0.997) within the test ranges. The established method showed good precision and overall recoveries of the boswellic acids.ConclusionsThe eight triterpenoic acids coded as BS-1 (11-keto-beta-boswellic acid), BS-2 (3-O-acetyl-11-keto-beta-boswellic acid), BS-3 (3-keto tirucallic acid), BS-4 (3-O-acetyl-alpha-tirucallic acid), BS-5 (3-O-acetyl-beta-tirucallic acid), BS-6 (alpha-boswellic acid), BS-7 (beta-boswellic acid) and BS-8 (3-O-acetyl-beta-boswellic acid) were isolated from the processed gum resin of Boswellia serrata by column chromatography. The proposed HPLC method is simple, reliable and has been very useful for the qualitative as well as quantitative analysis of boswellic acids in the gum resin of Boswellia serrata. The proposed method allows to quantify boswellic acids in appreciable amounts by HPLC-UV (DAD) method in the extracts and the available marketed formulations.Graphical abstractIsolation & separation of eight Triterpenoic acids from Boswellia serrata

Highlights

Boswellia serrata, known as Indian frankincense is a commercially important medicinal plant which has been used for hundreds of years as an Ayurvedic medicine for the attempted treatment of arthritis
There were reasonable differences in the retention times (Table 1) of said boswellic acids which facilitated the quantification of these acids in the samples
Separation and detection of pentacyclic triterpenic acids, consisting of six boswellic acids, namely, 11-ketobeta-boswellic acid, 3-O-acetyl-11-keto- betaboswellic acid, alpha-boswellic acid, beta-boswellic acid, 3-O-acetyl-alphaboswellic acid, and 3-O-acetyl-betaboswellic acid was carried out by Subbaraju et al whereas we were successful in the separation and detection of eight triterpenic acids including 3-keto-tirucallic acid, 3-O-acetyl-beta-tirucallic acid for the first time

Summary

Introduction

Known as Indian frankincense is a commercially important medicinal plant which has been used for hundreds of years as an Ayurvedic medicine for the attempted treatment of arthritis. It contains naturally occurring triterpenoic acids, called as boswellic acids (BA’s). Boswellin is methanolic/alcoholic extract of gum resin exudates of Boswellia serrata. Linn F (Burseraceae) is commonly used in Indian system of medicine (Ayurveda) as antiinflammatory, analgesic, anti-arthritic and anti-proliferative agent [1]. Clinical studies have revealed that Boswellia serrata plant may be effective in reducing diarrhoea in patients with inflammatory bowel disease [2]. Anti-inflammatory activity of ethanolic extract of Boswellia serrata is primarily due to inhibition of leukotriene synthesis.

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