Simultaneous Quantification of the Interplay Between Molecular Turnover and Cell Mechanics by AFM-FRAP.

Mark Skamrahl,Marco Fritzsche,Huw Colin‐York,Liliana Barbieri

doi:10.1002/smll.201902202

Mark Skamrahl, Marco Fritzsche + Show 2 more

Open Access

https://doi.org/10.1002/smll.201902202

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Abstract

Quantifying the adaptive mechanical behavior of living cells is essential for the understanding of their inner working and function. Yet, despite the establishment of quantitative methodologies correlating independent measurements of cell mechanics and its underlying molecular kinetics, explicit evidence and knowledge of the sensitivity of the feedback mechanisms of cells controlling their adaptive mechanics behavior remains elusive. Here, a combination of atomic force microscopy and fluorescence recovery after photobleaching is introduced offering simultaneous quantification and direct correlation of molecule kinetics and mechanics in living cells. Systematic application of this optomechanical atomic force microscopy-fluorescence recovery after photobleaching platform reveals changes in the actin turnover and filament lengths of ventral actin stress fibers in response to constant mechanical force at the apical actin cortex with a dynamic range from 0.1 to 10 nN, highlighting a direct relationship of active mechanosensation and adaptation of the cellular actin cytoskeleton. Simultaneous quantification of the relationship between molecule kinetics and cell mechanics may thus open-up unprecedented insights into adaptive mechanobiological mechanisms of cells.

Highlights

Understanding of their inner working and function
Recent research indi- retical model or numerical simulations for the analysis of the cates that they regulate their cell mechanics not exclusively molecular actions combined with knowledge of the recovery downstream of signaling events triggered by for instance time(s) of the respective molecule, the reaction kinetics and ligand–receptor binding, but that they employ a diversity of diffusion dynamics can be calculated and interpreted.[19,20,21]
This remarkable attribute goes reaction kinetics or diffusion dynamics or a combination of cells to modulate their mechanics involves biochemical of both processes.[13,22]

Summary

Introduction

Understanding of their inner working and function. Yet, despite the establishour understanding of the interplay of ment of quantitative methodologies correlating independent measurements of cell mechanics and its underlying molecular kinetics, explicit evidence and knowledge of the sensitivity of the feedback mechanisms of cells controlling their adaptive mechanics behavior remains elusive.

Results

Conclusion