Abstract
Simultaneous Quantification of T-Cell Receptor Excision Circles (TRECs) and K-Deleting Recombination Excision Circles (KRECs) by Real-time PCR.
Highlights
The clinical experience gathered throughout the years endorses primary immunodeficiency diseases (PIDs) awareness and guides research into newborn screening and future therapeutic strategies.Combined T-cell receptor excision circle levels (TRECs) and kappa-deleting recombination excision circles (KRECs) assay paves the way to new potential applications in this field
In an attempt to establish a reference range for TRECs and KRECs in healthy individuals with age ranging from 1 day-16 years, the 50 subjects included in this study were divided in to 5 different age groups and the following results were obtained as lower threshold of normal range for the studied parameters TRECs copies per ml of blood: 25172 for the group aged (1 day to 5 months); 23989 for the group aged (5 months to 2.4 years); 8294 for the group aged (2.4 years to 5.2 years); 8122 for the group aged (6 years to 9.5 years) and 4670 for the group aged(9.5 to 16 years)
In the work done by Sottini et al.[8] combined TRECs and KRECs quantification by real time PCR using triple insert plasmid dilutions as standard curve was performed in a representative sample of 42 children aged 0 - 16 years
Summary
Simultaneous Quantification of T-Cell Receptor Excision Circles (TRECs) and K-Deleting Recombination Excision Circles (KRECs) by Real-time PCR. Lamyaa Salem, Hala A Talkhan, Salwa Bakr, Afaf A. Clinical Pathology and Immunology Faculty of Medicine, Ain Shams University, Egypt
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