Simultaneous Quantification of Sinensetin and Tetramethoxyflavone in Misai Kucing Capsules using TLC-UV Densitometric Technique

Abstract

Thin-layer chromatographic (TLC)-UV densitometric method is developed for simultaneous quantification of sinensetin and 5-hydroxy--6,7,3', 4'-tetramethoxyflavone (TMF) in Misai kucing capsules (Orthosiphon stamineus). Sinensetin and TMF are isolated from the leaves of local Orthosiphon stamineus. The TLC-UV desitometric quantification is performed by external standard method on silica gel plates using chloroform-ethyl acetate (60:40) as developing solvent and UV detection at 365 nm.The TLC densitometer, although yields slightly higher values than the other analytical methods, is preferred due to its simplicity, easy and low cost. Keywords: Orthosiphon stamineus; Lamiaceae; Local medicinal plant; HPTLC; Quantification; Densitometer. © 2009 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved.DOI: 10.3329/jsr.v1i2.2300