Simultaneous quantification of phenolic acids and flavonoids in Chamaerops humilis L. using LC–ESI-MS/MS

Aicha Bouhafsoun,Ahmed Boukeloua,Mustafa Abdullah Yilmaz,Hamdi Temel,Meriem Kaid Harche

doi:10.1590/fst.19917

Abstract

Abstract In this study, heated reflux extraction method has been used to identify the phenolic compounds from C. humilis var. argentea leaflets, rachis and fruits. Extractions were performed in both ultrapure water and 80% methanol solvents. The efficiency of procedures was determined in terms of the quality and quantity of phenolic acids and flavonoids identified. Chamaerops extracts have been characterized by high concentrations of phenolic compounds, which play a crucial role in protection against various diseases. LC-MS/MS was used to determine the chemical profile of various extracts obtained from Chamaerops. The results showed that the major components in leaflets and fruits extracts were quinic, malic and chlorogenic acids. In addition, nine minor acidic components were identified. On the other hand, rutin and hesperidin were found to be the major flavonoids. The methanol extract was shown as being the most efficient to identify phenolic compounds in C. humilis.

Highlights

Flavonoids and phenolic acids, a class of polyphenol compounds, are widely distributed in plants kingdom
The objective of the present study was to characterize the chemical composition of water and 80% methanol extracts of C. humilis leaflets, rachis and fruits by using liquid chromatography coupled with mass spectrometry (LC-ESI-MS/MS) as a potent analytical technique
The variables considered during reflux extraction process including 80% methanol and ultrapure water were tested for the extraction of polyphenols from rachis, leaflets and fruits of Chamaerops, in order to achieve high extraction efficiency of phenolic acids and flavonoids

Summary

Introduction

Flavonoids and phenolic acids, a class of polyphenol compounds, are widely distributed in plants kingdom. Many studies reported that flavonoids exhibit various effects as antioxidant (Siahpoosh et al, 2016; Balci & Özdemir, 2016), anti-cancer (Androutsopoulos et al, 2010; Ma et al, 2015), anti‐allergic (Park et al, 2006), anti-thrombotic and vasodilatory (Rahimi et al, 2010), anticholinesterase (Ertas et al, 2016) actions. Because of their UV-absorbing properties, flavonoids protect plants from the UV radiation of the sun and scavenge UV-generated reactive oxygen species (Shirley, 1996). Previous studies reported Chamaerops to contain phenolic compounds, such as tannins, flavonoids, saponins, quinons, coumarines (Benahmed-Bouhafsoun et al, 2013), sterols and terpenoids (Hasnaoui et al, 2013)

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Conclusion