Abstract
A reversed-phase liquid chromatographic method was developed for the first time to simultaneously determine four major sesquiterpene lactones in Eupatorium lindleyanum, that is, eupalinolide A, eupalinolide B, eupalinolide C and 3β-acetoxy-8β-(4′-hydroxytigloyloxy)-14-hydxycostunolide. The chromatographic separation was achieved with a linear gradient of acetonitrile in water with a flow rate at 1.0 mL min−1 using an Alltima C-18 column (250 × 4.6 mm, 5.0 μm). Detection was carried out using a photodiode array detector at 210 nm. The calibration curves for the determination of all analytes showed good linearity over the investigated ranges (r2 > 0.999). Repeatability was evaluated by intra-day and inter-day assays and RSD values were less than 2.7%. The recoveries were between 94.0 and 104.9%. This developed method can be used for evaluate the quality of different parts of E. lindleyanum.
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